Rotenone toxicity treatment
Rotenone toxicity treatment. Nov 28, 2022 · Rotenone is a commonly used insecticidal chemical in agriculture and it is an inhibitor of mitochondrial complex Ⅰ. Rotenone treatment at 250 nM reduced cell viability by approximately 50%. Furthermore, the toxicity of rotenone on dopaminergic nigrostriatal neurons and peripheral tissues was Rotenone is considered moderately toxic according to the World Health Organization, with an LD 50 of 132 mg/kg of body weight and its toxicity depends on the ability to inhibit cellular respiration by blocking mitochondria in complex I (World Health Organization 1992; Chauvin et al. 5 mg/kg body weight) led to a reduced body weight compared to the control group. Rotenone exerts selective toxicity, as it is highly toxic to fish because of its rapid absorption from the GI tract in comparison with mammalian species in which it is poorly absorbed. 5 or 4. As with most food-borne poisoning events, cases may be underreported. 2008). Selective toxicity of rotenone to insects and fish in comparison to mammalian species is due to greater absorption from the GI tract and formation of large quantities of highly toxic metabolites. Oct 5, 2015 · Taurine treatment produced a concentration-dependent reduction in rotenone-induced cell death. , 2000; Sherer et al. Rotenone is a pesticide and a piscicide derived from the derris root. Treatment relies on symptomatic and supportive hours, and rotenone degrades quickly through a variety of environmental and metabolic mechanisms. There is no specific antidote available for rotenone or rotenoids. Rotenone, a potent mitochondrial complex I inhibitor (Xiong et al. Dimethyl sulfoxide (DMSO) was provided by Solarbio (Beijing, China). Jun 2, 2022 · Collectively, our results reveal that GDF15 reduces SH-SY5Y cell apoptosis by decreasing ROS generation. Mar 27, 2008 · Co-treatment with centrophenoxine (100 mg/kg i. From this, we tested sub-effective concentrations of taurine in combination with low, sub-effective concentrations of NAC against rotenone toxicity, and found the combined treatment afforded greater cytoprotection than either treatment alone. T … Jul 14, 2016 · There is no specific antidote, but human poisoning of rotenone can be managed by gastric lavage and treatment with activated charcoal, N-acetyl cysteine, antioxidants (tocopherol and coenzyme Q 10) and potassium channel-opening drugs such as iptakalin and diazoxide . Rotenone kills fish not by removing oxygen from the Nov 29, 2022 · After 2 h of rotenone incubation, increased ROS production and altered Δψm were observed, hours later resulting in DNA oxidative damage and apoptosis. Jan 1, 2021 · 1. 2007). However, poisoning with commercially available rotenone in humans has been reported only once previously following accidental ingestion in a 3. 19 µm/s (p = 0. Rotenone treatment (6–8 hr) mildly depleted cellular ATP levels (32. No changes in rotenone-induced toxicity were observed with or without AMPT treatment. We found that rotenone poisoning is rare but can be fatal. Moreover, a rapid method to detect rotenone poisoning is not available. Mechanistically, rotenone inhibits mitochondrial complex I, leading to reduced ATP production, compensatory glycolytic upregulation and secondary lactate production, and oxidative stress. Rotenone treatment (6-8 hr) mildly depleted cellular ATP levels (32. Our literature review examined acute rotenone poisoning in humans immediately or shortly after exposure to Rotenone: * Rotenone can irritate the skin causing a rash or burning feeling on contact. Oxidative stress is one of the main contributors of neurodegeneration in PD. 5 μg L-1) and duration (6-hour) used during the smallmouth bass Micropterus dolomieu eradication in the Rondegat River South Africa [25, 26, 30]. 1 A and and3 3 D). Apr 4, 2024 · To our knowledge, this study is the first to comprehensively summarize acute rotenone poisoning in humans and to describe associated treatments and consequences. 75 and 7. 0-5. However, more and more studies have reported that rotenone has high toxicity to domestic animals, fish, silkworm and mice, especially neurotoxicity and reproductive toxicity 4-8. After 48 h of treatment, we observed that rotenone exhibited cytotoxicity in a concentration-dependent manner from 10 to 1000 nM . p. A suicide by rotenone poisoning in an adult is described. Commercial rotenone products are classified as RUP due to known acute inhalation, acute oral and severe aquatic toxicity, resulting in classification as a category IV toxicant (USEPA, 2007). Diagnosis of rotenone poisoning is based on detection of rotenone residue in blood, liver, urine, feces, and vomitus. Apr 4, 2017 · The treatment of male rats with 400 ppm rotenone through the diet (resulting in a daily intake of 52. , 2012), is a hydrophobic compound that easily traverses the blood brain barrier causing disorders in the central nervous system. Jul 26, 2017 · Rotenone treatments were used in the East Fork of Specimen Creek (EFSC) drainage during 2006-2009 to remove non-native trout. Previous studies have found that rotenone induces the production of reactive oxygen species (ROS) by inhibiting electron transport in the mitochondria of somatic and germ cells. Pathogenic, teratogenic, carcinogenic and anti-cancer effects 21 9. Oct 29, 2012 · Rotenone has several characteristics that make it desirable as a tool to sample fish and eradicate invasive fish species. The mechanism for the cytotoxicity is at mitochondrial level affecting cellular respiration. 5% at 10nM) (Fig. May 11, 2020 · Rotenone, known as an inhibitor of mitochondrial complex I, is a toxic rotenoid compound extracted from leguminosae family plants and can be used as botanical insecticide. Environmental persistence 13 6. 1,2,12,12a-Tetrahydro-8,9-dimethoxy-2-(1-methyl-ethenyl)-[1]benzopyrano [3,4-b]furo[2,3-h][1] benzopyran-6(6aH)-one Colorless to red, odorless, crystalline solid. Rotenone has been used on humans for external treatment of chiggers (2% lotion) and scabies (10% emulsion). May 28, 2020 · 2. 3A). 9% decrease at 100 n m; 15. Nov 8, 2018 · The percent of mitochondria moving per cell remained unaffected by the 10 mg/mL ascorbic acid treatment averaging 98–100% moving (Figure 8 A), though the ascorbic acid treatment increased velocity 55% beyond the increase already induced by rotenone from 0. The experimental design included rotenone treatment concentrations (50 and 37. However, the EPA does recommend precautionary measures such as wearing protective clothing for the applicators and closing water bodies treated with rotenone to swimming for a few days following treatment. 0 mg/10 g diet of TA to understand its protective capacity on rotenone (500 μM)-induced toxicity after 7 days of treatment as follows: Group 1 (Control Nov 11, 2003 · Rotenone toxicity does not result solely from ATP depletion. , 2018). 4 First-aid measures and management principles Ingestion: Induce vomiting unless the product vehicle is a petroleum distillate (see the treatment protocol on decontamination procedures) Gastric lavage may be performed if a large amount of rotenone was ingested even if it is dissolved in kerosene or a related petroleum distillate. Treatment of Rotenone Toxicity in Dogs Your veterinarian will continue to induce vomiting by performing gastric lavage and administering activated charcoal, if rotenone has been ingested. A slurry of Apr 15, 2024 · A specific rotenone antidote does not exist and treatment for rotenone poisoning is purely supportive. ) to determine if the drug could prevent rotenone-induced damage to the central nervous system (CNS). Measuring rotenone concentrations in water and other materials 12 5. Introduction. The use of rotenone poses serious challenges to conservation practitioners due to its impacts on non-target organisms including amphibians and macroinvertebrates. 09 ± 4. 5-year-old child. 1, 2 The pesticide effect of rotenone is relatively non-toxic or moderately toxic to humans. for 30-45 days) in mice aged 2. 003 µm/s to 0. However, there is little precise information on the effects of rotenone exposure in porcine oocytes Sep 24, 2018 · Mechanism of Rotenone Toxicity in Catecholaminergic Neurons. Jan 1, 2003 · Rotenone treatment of streams in Papua New Guinea caused immediate. 3 The toxic effect in man was described in a remote tribe who habitually committed suicide by eating derris root. The SH-SY5Y cells were classified into three groups: the control group, the ROT-induced lesion group (20 μM), and the PPVI treatment group (concentrations of 3. May 1, 2024 · Rotenone (ROT, 95 % purity, CAS: 83–79-4) was sourced from BD Biosciences (Shanghai, China). Using laboratory studies, we investigated the effects of different rotenone concentrations (0, 12. Detoxifying rotenone in water and the use of fish antidotes 22 10. Similar results were Fish may be killed by using rotenone, a chemical compound extracted from the roots of certain species of the bean family. The concen-tration of active rotenone in liquid formulations is normally 2. The World Health Organization classifies rotenone as moderately hazardous because it may be absorbed by ingestion or inhalation. 8 , 9 Recent Apr 5, 2016 · However, when LiCl was pre-added 72 hours before rotenone, the activity of caspase-3 was down to 141. Apr 12, 2012 · Rotenone is one of most acutely toxic botanicals with LD50 (lethal dose that kills 50% of the test population of rats) ranges from 132 to 1,500 mg/kg with variability due to differences in plant extracts. 2 ± 2. Rotenone residue can be determined using high performance liquid chromatography coupled with a fluorescence detector or liquid chromatography–tandem mass spectrometry. 5, 50, 100 μg L-1 differentiate between rotenone active ingredient and commercial formulation concentrations. Of particular interest, high-grade ming is not allowed in rotenone-treated water until the application has been completed and all the rotenone has been thoroughly mixed into the water. Rotenone shampoo can be used to control mites on dogs and cats, but alternative methods of treatment are often used. Flavonoids have the potential to modulate neuronal function and combat various neurodegenerative diseases. Rotenone is safe for all livestock in watering tanks except for swine. The pesticide rotenone was discovered to act via electron transport chain (ETC) complex I inhibition in the early 1960s (Lindahl and Öberg 1961). Metabolic fate of rotenone in higher animals (birds and mammals) 21 8. m. H&E staining of liver tissues showed no difference between groups in morphology (Figure 6A). If skin exposure is noticeable or if you have not had the chance to do this yourself, the veterinarian would bathe your dog in warm water and soap, rinsing it Rotenone toxicity involved oxidative damage to proteins, showed PD-related motor deficits (Fleming et al. We evaluated the cell viability and cytotoxicity and apoptosis rates of SH-SY5Y cells treated with rotenone and probucol or edaravone, a known free-radical scavenger. May 28, 2020 · Concerning protein expression propionic acid treatment increased STAT3 levels but did not lead to an increased phosphorylation indicative of pathway activation. It is particularly toxic to fish and other gill breathing organisms as it can easily enter the blood unchanged through the thin tissue of the gills Rotenone does not cause cancer, birth or reproductive defects and does not cause unreasonable Mar 17, 2022 · From the data obtained above, we selected 1. It was used as a broad-spectrum insecticide and a fish poison, but is now banned in many countries due to its toxicity and environmental impact. These results showed that GDF15 overexpression could prevent rotenone 4. 7 Studies have shown that rotenone plays an important role in regulating Parkinson's disease through inducing the death and oxidative damage of nerve cells. 5% or 5%, and a treatment rate of 1 and 3 mg/L (ppm) formulation (5% active) would yield active rotenone concentrations of 50 and 150 lg/L, respectively. 45 ± 0. 5% at 10 n m) (Fig. 5, 25, 37. Mar 21, 2024 · Supportive treatment addressed symptoms and included gastric lavage and/or activated charcoal to remove rotenone from the stomach, vasopressors for hypotension, mechanical ventilation for respiratory insufficiency, and sodium bicarbonate for acidosis. In vitro cell studies have shown that rotenone-induced toxicity is reduced by the use of N -acetylcysteine, antioxidants and potassium channel openers. Jan 1, 2020 · While previously treated as an organic treatment, more recently, rotenone use has been regulated. The incidence of rotenone poisoning is unknown, but it is generally considered an infrequent event. Thus, most physicians will not have encountered a patient. Contact your veterinarian if your pet experiences drooling, vomiting, diarrhea, changes in breathing rate, muscle twitching, seizures or coma while being treated with a rotenone-containing compound. Rotenone, used as a piscicide, is a restricted use chemical, and a license is required for its purchase and application. Rotenone poisoning can be fatal and there is no antidote. To further investigate Wnt/β-catenin signaling in rotenone-induced cell injury, we examined Wnt3a, β-catenin and non-phospho-β-catenin by Western blotting. Rotenone toxicity has been studied using various in vitro and in vivo models (reviewed in Xiong et al. Baicalin treatment at 50 µM counteracted rotenone toxicity by modulating the expression levels of some proteins involved in mitochondrial biogenesis and apoptosis. Rotenone is a colorless, odorless, crystalline isoflavone that occurs in some plants. * Breathing Rotenone can irritate the nose, throat and lungs. This mechanism of action became of particular interest to the Parkinson’s disease (PD) research community after it was discovered that there was significant complex I inhibition in parkinsonism induced by 1-methyl-4-phenyl-1,2,3,6 Nov 5, 2015 · Rotenone, a naturally occurring ketone, is widely employed for the management of invasive fish species. Pure rotenone is moderately toxic for humans, but since the concentration of active ingredient in many marketed formulations is low, the use of this pesticide has resulted in few intoxications. 2001). , (2009) have reproduced consistently many features of PD in rats following chronic intraparitoneal administration of rotenone. was applied Oll January 5, 195;;. It has been used in numerous lakes and reservoirs in Oregon with great success to remove unwanted fish species and restore traditional fisheries. These fish likely preyed on the tadpoles and suppressed their numbers. Rotenone residue is also likely to be present in the liver. Twenty-four hours later, cages containing fathead minnows were placed in the ponds to check the rotenone toxicity at this low temperature. Complex I inhibition after rotenone exposure can have several cellular consequences, including depletion of cellular ATP. (Fig. c. Diamond Lake was treated in 1954 with rotenone and treatment was 100 percent successful. Rotenone treatment decreased the number of dopaminergic neuronal cells in the substantia nigra pars compacta (SNpc) and lesioned nerve terminal in the striatum. Jun 16, 2024 · We first confirmed the toxicity of rotenone, an inhibitor of mitochondrial complex I, to SH-SY5Y cells. Rotenone is an alkaloid pesticide and piscicide derived from the derris root or stem. A specific rotenone antidote does not exist and treatment for rotenone poisoning is purely supportive. rotenone at a concentration of 0. Feb 21, 2017 · The marked increase in tadpoles following the rotenone treatment was likely a result of the absence of trout in High Lake, as the rotenone treatment removed over 800 trout from the lake, some as large as 400 mm in length (Koel et al. p for 21 days) to induce PD-like pathology and were co-treated with increasing dosages of buspirone (1, 3, or 10 mg/kg i. 0131) (Figure 8 B). In order to evaluate the hepatotoxicity of rotenone at the dose used in this study, we compared some indexes of liver function in the mice with or without rotenone treatment. This treatment killed fish stranded in these ponds within 24 hours. Apr 1, 2018 · The addition of rotenone (60 μM, 24h) to the cell culture medium reduced the cell viability to approximately 50% in the WT+Rotenone-group and the Vehicle+Rotenone-group, while treatment with rotenone on SIRT3+ cells caused the cell viability to decrease approximately 30%, indicating that SIRT3 overexpression preserved cell viability (Fig. 0 ± 2. Rotenone is the most commonly used compound for treating lakes. Indeed, rotenone treatment caused low-grade mROS production (Fig. , 2004) and Lewy body-like inclusions that immunostained for α-synuclein and ubiquitin were observed in the substantia nigra and striatum (Betarbet et al. Jan 15, 2019 · Evaluation of rotenone toxicity in the liver of mice. Feb 15, 2007 · Therefore, we investigated the effects of chronic exposure to rotenone (2. The first treatment occurred in 2006 at the upper end of the drainage, which included High Lake and its outlet stream, ending at a waterfall barrier (Koel et al. May 4, 2020 · The rotenone-treated mice exhibited motor deficits assessed by open field, rotarod and cylinder test and gastrointestinal dysfunction. 4 A), but the latter was not sufficient to activate the NLRP3 inflammasome (Figs. The water temperature on that date of 52° F. There is no specific antidote 2. Apr 29, 2005 · Poisoning with rotenone is uncommon but is potentially fatal because this agent inhibits the mitochondrial respiratory chain. Nov 21, 2023 · Treatment with 10 and 20 μM of oxyresveratrol effectively prevented rotenone-induced cell death as exhibited by an increase of approximately 14% and 28%, respectively, compared to the rotenone group. 0 mg/kg s. Therefore, the optimal management of Dec 31, 2009 · Although some researchers [17,18] have reported non-specific central nervous system and systemic toxicity in rats upon rotenone treatment, recently Cannon et al. Nov 12, 2021 · The rotenone-induced animal model of Parkinson's disease (PD) has been used to investigate the pathogenesis of PD. Thus, in this study, we investigated whether probucol can alleviate the effects of a mitochondrial complex I inhibitor, rotenone, on a human neuroblastoma cell line (SH-SY5Y). During the treatment period, the effects on vitality and motor behavior were investigated. p. Nov 5, 2015 · In addition, a no rotenone (0 μg L −1) treatment was employed using the same filtered water which served as a control. * Rotenone can irritate the eyes on contact. Nov 29, 2011 · Rotenone FAQ's. Poisoning following deliberate ingestion of these plant roots has commonly been reported in Papua New Guinea. 10 p. , 2003). Emulsifiable concentrates and wettable powders of rotenone are also extensively used in lakes, ponds, and reservoirs to control undesirable fish. Jul 4, 2022 · Rotenone was originally thought to be very safe for humans and animals, in addition to being toxic to insects and aquatic animals. catastrophic invertebrate drift, especially mayflies, but did not cause large-scale rotenone toxicity but illustrate that . Features include systemic mitochondrial impairment Oct 1, 2011 · 1. Rotenone Treatment Reduced the Neurite Outgrowth and Amount of Dopaminergic Cells. 01) in comparison to rotenone treatment alone. 3A). An innovative laboratory methodology was developed for the principal requiremen … Apr 29, 2005 · Rotenone is a pesticide derived from the roots of plants from the Leguminosae family. Mar 22, 2024 · Rotenone is a toxic chemical found in various plants, including some used as food. The toxicity of rotenone 13 7. 1. Rotenone, also known as derris or cube, is used as an insecticide as well as a piscicide (fish poison). Our findings indicate that both Fasudil and propionic acid treatment show beneficial potential in rotenone-lesioned primary mesencephalic cells. for 35 days) significantly attenuated the extent of motor dysfunction and changes in the level of dopamine and lipid peroxidation induced by rotenone toxicity. Interestingly a 2 mg To determine whether endogenous DA makes PC12 cells more susceptible to rotenone, cells were treated with the tyrosine hydroxylase inhibitor alpha-methyl-p-tyrosine (AMPT) to reduce DA levels prior to rotenone exposure, and then cell viability was measured. , 2012; Terron et al. MMP was assessed by JC-10 assays, and this was significantly decreased by rotenone treatment, whereas GDF15 could mitigate the toxic effect of rotenone (Figure 4A). Similar results were observed Feb 6, 2022 · To test if buspirone elicited neuroprotective activities, C57BL/6 mice were subjected to rotenone treatment (10mg/kg i. Re-strict swine from the water source until the rotenone has been detoxified. Rotenone toxicity does not result solely from ATP depletion. 9% decrease at 100nM; 15. 5 μM). 27 ± 0. * Exposure to Rotenone can cause nausea, vomiting, abdominal cramps, headache, muscle tremors, convulsions Nov 6, 2015 · Previous studies focused on mROS production by rotenone-mediated complex I inhibition in order to explain the toxic effect of rotenone (16, 17). Chief among these characteristics are that it degrades quickly in the environment and from fish tissue (often on the order of days), it does not bioaccumulate, and it is not as toxic to birds, mammals or aquatic insects as it is to fish. 4 Human fatalities are rare, perhaps because rotenone is available in weak1% to 5% 4. 67% (P < 0. In order to define a rotenone toxicity experimental paradigm, we caused oxidative cellular stress by application of different rotenone concentrations on primary mesencephalic neurons. 5, 5, or 12 months. nqijs lubb ftllwp zadere zsolrw nkfk zpedxin ntv rjrd szbba